Constitutive and stimulated cytokine production by primary type II alveolar epithelial cells
| Stimulant | ||||||
|---|---|---|---|---|---|---|
| Basal | Staphylococcus aureus PGN | S. aureus LTA | Pseudomonas aeruginosa LPS | CpG | TNF | |
| IL-1β (pg/mL) | 5 2.5–8 | 17.2 ** 5–152 | 3.4 1.6–12.5 | 6.3 2.2–14 | 7.5 1.7–11 | 11 1.2–85.3 |
| IL-6 (pg/mL) | 236 8.3–1276 | 927 * 121–9060 | 333 7.6–716 | 214 8.2–533 | 228 12.6–803 | 1205 ** 34.1–4029 |
| IL-8 (pg/mL) | 2273 707–11 226 | 7444 * 1283–100 000 | 2002 843–21 914 | 1507 649–13 548 | 2919 636–40 775 | 31 721 *** 9450–78 198 |
| IL-10 (pg/mL) | 15 2.6–1276 | 25.4 ** 3.5–5000 | 23.2 3.6–716 | 19.2 3–504 | 20.2 0–803 | 26 * 3.5–4029 |
| IL-12 (pg/mL) | 8 5.4–19.7 | 7.3 6.6–31.2 | 8.3 4.9–30 | 12.7 3.5–25 | 12.0 2.7–28.6 | 7 2.7–30.3 |
| TNF (pg/mL) | 10 3.6–21.2 | 29 * 6.5–279 | 5 0–21.4 | 12 2.3–26.7 | 7.0 0–15.7 | † |
Data are expressed as median (upper line, italic) and range (lower line, normal text). n=7 for all conditions. PGN and LTA were applied at 10 μg/mL, LPS at 100 ng/mL, CpG at 1 μM and TNF at 10 ng/mL. Statistical analysis was by Friedman's test and Dunn's post hoc test. *p<0.05, **p<0.01, ***p<0.001 relative to basal levels, by Dunn's post hoc test.
†TNF was used as a positive control; TNF was not measured in TNF-stimulated cells.
IL, interleukin; LPS, lipopolysaccharide; LTA, lipoteichoic acid; TNF, tumour necrosis factor; PGN, peptidoglycan.