Table 1

Analysis of relative protein level and phosphorylation status of ERK 1/2 and AKT in lung and tumour tissue

	Lung vs matched tumour (±SD)			Squamous cell carcinoma vs adenocarinoma (±SD)			Emphysema vs histologically normal lung (±SD)
	Lung	Tumour		Squamous	Adenocarcinoma		Emphysema	Normal
Measured kinase	n=19	n=19	p Value*	n=10	n=9	p Value†	n=13	n=7	p Value†
Total ERK (RU)	17 179±8222	18 578±9886	0.75	19 815±10 399	17 298±9099	0.65	18 836±8147	14 997±9638	0.51
Total ppERK (RU)	2630±912	1170±355	0.006	1236±506	977±239	0.67	4159±1722	853±422	0.001
ppERK as per cent total ERK	19.1±11.2	8.8±8.6	0.002	8.3±6.3	9.3±11.1	0.80	23.5±9.2	8.3±9.0	0.002
Total AKT (RU)	6457±2673	25 645±10 447	0.0001	27 040±11 803	24 155±8750	0.79	7079±2748	6026±2780	0.70
Phospho-AKT (RU)	1932±511	10 186±3327	0.001	9204±3020	11 402 ±3516	0.69	2223±557	1706±490	0.68
pAKT as per cent total AKT	33.2±13.5	48.6±13.0	0.001	48.7±15.2	48.4±11.0	0.96	34.3±12.7	31.7±14.9	0.69