Article Text

Download PDFPDF

Face mask sampling reveals antimicrobial resistance genes in exhaled aerosols from patients with chronic obstructive pulmonary disease and healthy volunteers
  1. Matthew Kennedy1,2,
  2. Mohammadali Y Ramsheh1,
  3. Caroline M L Williams1,
  4. Joss Auty1,
  5. Koirobi Haldar1,
  6. Mohamad Abdulwhhab1,
  7. Christopher E Brightling1,2 and
  8. Michael R Barer1,2
  1. 1 Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK
  2. 2 Department of Clinical Microbiology, University Hospitals of Leicester, Leicester, UK
  1. Correspondence to Dr Matthew Kennedy; mk526{at}le.ac.uk

Footnotes

  • Contributors MK: study conceptualisation, data collection, laboratory processing of COPD and volunteer samples, data interpretation, statistical analysis and drafting of manuscript. MYR, CMLW and JA: assistance with laboratory processing of samples. MA: collection of volunteer samples and extraction of DNA. KH: assistance with laboratory processing of samples and manuscript review. CEB: project supervision. MRB: study conceptualisation and manuscript drafting and review.

  • Funding This work was supported by a grant from the Dowager Countess Eleanor Peel Trust and the UK MRC (MR/P023061/1).

  • Competing interests None declared.

  • Patient consent Obtained.

  • Ethics approval Ethical approval was obtained from the East Midlands Research Ethics Committee (REC) approval 08/H0406/189. Additionally, aerosol DNA extracts were available from 10 healthy volunteers previously recruited at the University of Leicester between September and October 2015 (departmental ethics committee approval: 2369-ma680-3i).

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data sharing statement All data collected as part of this study and used to reach the above conclusions are included within this article and in the online supplementary material. Data displayed within this article are available upon request from MK.

View Full Text

Statistics from Altmetric.com

Footnotes

  • Contributors MK: study conceptualisation, data collection, laboratory processing of COPD and volunteer samples, data interpretation, statistical analysis and drafting of manuscript. MYR, CMLW and JA: assistance with laboratory processing of samples. MA: collection of volunteer samples and extraction of DNA. KH: assistance with laboratory processing of samples and manuscript review. CEB: project supervision. MRB: study conceptualisation and manuscript drafting and review.

  • Funding This work was supported by a grant from the Dowager Countess Eleanor Peel Trust and the UK MRC (MR/P023061/1).

  • Competing interests None declared.

  • Patient consent Obtained.

  • Ethics approval Ethical approval was obtained from the East Midlands Research Ethics Committee (REC) approval 08/H0406/189. Additionally, aerosol DNA extracts were available from 10 healthy volunteers previously recruited at the University of Leicester between September and October 2015 (departmental ethics committee approval: 2369-ma680-3i).

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data sharing statement All data collected as part of this study and used to reach the above conclusions are included within this article and in the online supplementary material. Data displayed within this article are available upon request from MK.

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.