Rationale The airway microbiota is important in chronic suppurative lung diseases, such as primary ciliary dyskinesia (PCD) and cystic fibrosis (CF). This comparison has not previously been described but is important because difference between the two diseases may relate to the differing prognoses and lead to pathological insights and potentially, new treatments.
Objectives To compare the longitudinal development of the airway microbiota in children with PCD to that of CF and relate this to age and clinical status.
Methods Sixty-two age-matched children (age range 0.5–17 years) with PCD or CF (n=31 in each group) were recruited prospectively and followed for 1.1 years. Throat swabs or sputum as well as clinical information were collected at routine clinical appointments. 16S rRNA gene sequencing was performed.
Measurements and main results The microbiota was highly individual and more diverse in PCD and differed in community composition when compared with CF. While Streptococcus was the most abundant genus in both conditions, Pseudomonas was more abundant in CF with Haemophilus more abundant in PCD (Padj=0.0005). In PCD only, an inverse relationship was seen in the relative abundance of Streptococcus and Haemophilus with age.
Conclusions Bacterial community composition differs between children with PCD and those with CF. Pseudomonas is more prevalent in CF and Haemophilus in PCD, at least until infection with Pseudomonas supervenes. Interactions between organisms, particularly members of Haemophilus, Streptococcus and Pseudomonas genera appear important. Study of the interactions between these organisms may lead to new therapies or risk stratification.
- cystic fibrosis
- paediatric lung disaese
- respiratory infection
Data availability statement
Data are available in a public, open access repository. Sequence data is available at the ENA Accession number: PRJEB26618.
This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.
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The airway microbiota is highly individual and differs between PCD and CF. Study of the different interactions between components of the microbiota, especially Haemophilus, Streptococcus and Pseudomonas, may potentially lead to new therapies.
This is the first study to describe the longitudinal development of the airway microbiota in children with PCD, and how it differs from CF to help to further our understanding of microbiological processes when bacterial elimination is compromised.
Airway infections in chronic suppurative lung diseases (CSLD), such as primary ciliary dyskinesia (PCD) and cystic fibrosis (CF), are important. Previously, based on bacterial cultures, it was suggested that airway infections in CSLD are caused by only a handful of pathogens. Bacterial sequencing, however, has identified rich bacterial communities (‘microbiota’) in the airways of patients with CSLD. This includes ‘traditional’ pathogens, such as Pseudomonas aeruginosa, as well as many other micro-organisms particularly anaerobes. The role of these polymicrobial communities in disease progression is at present unclear.1–3
The relationship between airway bacterial diversity and disease severity has received much attention. CF has been relatively well studied, with several longitudinal studies in adults and large, cross-sectional studies across all ages. These have described an inverse relationship between diversity and spirometry (forced expired volume in 1 s (FEV1)) with an increase in diversity seen at least until school age4 and a decrease in adulthood.5–11 Antibiotic use was found to be a primary driver of reduced diversity in adults,8 suggesting perhaps antibiotics should be used more judiciously. Several longitudinal studies of the airway microbiota in infants with CF have however demonstrated either a decrease12 13 or no change in diversity with age.14–16 In infants and preschool aged children, diversity was not associated with FEV1 at 6 years13 or antibiotic usage.12 Whether reduced diversity is a marker of end-stage disease, an effect of the frequent courses of antibiotics that many patients with CF receive, or an innocent bystander, has yet to be determined.
Longitudinal studies in infants with CF have demonstrated changes in community composition with age.14 17 Previously, we described a significant inverse relationship between Streptococcus and Haemophilus in oropharyngeal samples until 2 years of age in infants with CF diagnosed on newborn screening (NBS).16 Similarly, a comparison of bronchoalveolar lavage (BALF) between clinically stable children with CF and controls aged between one and 6 years of age demonstrated a trend towards an increase in the abundance of Proteobacteria (which includes Pseudomonas, Neisseria and Haemophilus) and a significant decrease in Streptococcus and other anaerobes with age in CF.12 The clinical significance of this relationship is unknown but there is evidence of a significant reduction in airway inflammation (BALF total cell and neutrophil counts) with an increased relative abundance of anaerobes Streptococcus, Veillonella and Prevotella.18 Due to high interpatient variability,6 18 longitudinal studies coupling FEV1 with diversity of the airway microbiota in children have the potential to clarify this relationship.
The airway microbiota is thought to be governed by three factors: bacterial immigration (eg, through microaspiration from the oropharynx and seeding from the upper airways), elimination (eg, through mucociliary clearance) and regional growth conditions.19 PCD is caused by genetically inherited dysfunction of motile cilia. It shares certain pathological features with CF including impaired mucociliary clearance, chronic airway infections and neutrophilic airway inflammation.20 Important differences, however, exist between these two conditions, including differences in sputum composition, which may influence regional growth conditions and subsequent airway microbiology. Haemophilus influenzae is frequently cultured in CF and PCD, but Streptococcus pneumoniae is more prevalent in PCD while P. aeruginosa is more prevalent in CF.21 PCD however, for unknown reasons, rarely leads to as steep a decline in spirometry as CF (FEV1 annual decline 0.8% in PCD and 3.6% in CF21). There is much less information about the longitudinal development of the microbiota in PCD. To date there is only a single cross-sectional published study of the microbiota in 24 patients with PCD across a wide age range (between 4 and 73 years of age).22 Understanding the variation in the microbiota between these two forms of CSLD is important to further our understanding of microbiological processes when bacterial elimination is compromised and may lead to development of novel therapies.
In this study, we used sputum or throat swabs (TS), the latter which we have previously confirmed are a surrogate for the lower airway,23 to describe the airway microbiota longitudinally in children with CSLD. We hypothesised that the microbiota would differ between PCD and CF and would show differences between the two disease with age and clinical status.
Subjects and sampling
Children were recruited and followed up opportunistically during routine clinical appointments (on average every 6 months for PCD and 3 months for CF) at the Royal Brompton Hospital (RBH) between December 2012 and March 2014. The inclusion criteria were regular attendance at RBH for clinical appointments and diagnoses of PCD and CF made in accordance with standard guidelines24–26 (see online supplemental file 1). The exclusion criteria were patients who were receiving CFTR modulating medications (of which only ivacaftor was available at the time of the study).
Sputum samples, or TS in non-expectorating patients, were collected alongside detailed clinical information and handled as previously described (see online supplemental file 1 and online supplemental table S1).23 Where possible, paired sputum samples and TS were collected to compare the microbiota between these sample types. Technical control samples using blank swabs were collected to test for contamination at the point of sampling. Simultaneous microbial culture was performed as per standard clinical practice according to the CF Trust Guidelines27 in the Clinical Microbiology Department at RBH.
Pulmonary exacerbations were defined as a change in clinical features necessitating treatment with intravenous antibiotics as determined by an independent clinician. P. aeruginosa chronic status was defined according to the Leeds criteria.28
Patients or the public were not involved in the design, conduct, reporting or dissemination of our research.
16S rRNA gene library preparation and sequencing
For DNA extraction, whole sputum samples were defrosted and 300 µl added directly to a Lysing Matrix E (LME) tube. For TS, frozen swab heads were transferred directly to an LME tube containing sodium phosphate buffer. Further DNA extraction steps, 16S rRNA gene V4 region library preparation and sequencing (using the Illumina MiSeq V2 reagent kit) were performed as described previously.23
Sequence processing was performed in Quantitative Insights into Microbial Ecology (QIIME) V.22.214.171.124 After combining forward and reverse reads, de-multiplexing, quality filtering and removal of PhiX, 35 923 028 reads were obtained with a median of 18 324 reads per patient sample (range 82–348 657 reads). Operational taxonomic unit (OTU) picking was performed in UCLUST (V.1.2.22q) with a threshold of 97% sequence similarity.29 Undibacterium spp, Comamonadaceae and an individual Chlamydophila OTU (ID 4987) were found to be highly abundant in control samples and were removed as contaminants30 (online supplemental figure S1). Samples were rarefied to 1000 reads to standardise sequencing depth resulting in 76 (18.6%) of the 409 samples being removed prior to further data analysis (online supplemental figure S2). The composition of mock communities was compared with ensure sequencing consistency between sequencing runs (online supplemental figure S3).
Downstream analyses were performed using Phyloseq (V.1.20.0)31 in R (V.3.4.0).16 Barplots were constructed using ggplot2 (V.2.2.1)32 and gridExtra (V.2.2.1).33 Non-linear mixed effects modelling using a negative binomial distribution was performed using glmmADMB (V.0.8.3.3)34 controlling for patient to assess the relationship between age range (1–2 years, 2–3 years, etc) and (a) alpha (within subject) diversity, measured by richness, evenness, Shannon and Inverse Simpson’s diversity indices (see online supplemental file 1) and (b) the relative abundance of the most common genera.
Beta (between subject) diversity differences (Bray Curtis dissimilarity) were tested using a permutational multivariate analysis of variance (PERMANOVA)35 blocked by participant study number, using the adonis function in vegan (V.2.4.3).35 OTU level changes were assessed using multiple correlation testing using Spearman’s rank with a false discovery rate correction (Benjamini-Hochberg) and adjusted p values reported.
To assess the relationship between clinical variables (online supplemental table S1) and alpha diversity, t-tests and Wilcoxon signed-rank tests were used for parametric and non-parametric binary variables and ANOVAs and Kruskal-Wallis test similarly for variables with multiple values. Pearson correlation was used to test changes in continuous variables and alpha diversity. A PERMANOVA was used to test the relationship between beta diversity and clinical variables within each patient group. All variables were initially tested independently to determine which were significant. Age was analysed as a categorical variable (as age ranges, see above).
Significant variables were then tested in a combined model. For CF, the variables included in the final model were: P. aeruginosa chronic infection status at baseline, growth of P. aeruginosa during the study, azithromycin prophylaxis and treatment courses (oral co-trimoxazole, nebulised colomycin and nebulised tobramycin). For PCD, the variables included were gender and H. influenzae growths. A p value <0.05 was considered statistically significant. Sequence data is available at the ENA Accession number: PRJEB26618.
Agreement between microbial cultures and 16S rRNA gene sequencing was assessed by comparing the frequency with which organisms grown on culture were also the most abundant organism identified by molecular techniques and calculating Cohen’s kappa statistic.
Each disease group contained 31 children. Baseline demographics are given in table 1. Follow-up was for a mean of 1.1 years for both groups (SD 0.48 and 0.46 years, PCD and CF, respectively). Medians of five (PCD, range 2–15) and seven (CF, range 2–23) samples per subject were collected (online supplemental figure S2 and S4).
Clinical microbiological cultures during the study period differed between PCD and CF (table 2). S. pneumoniae (p=0.01) and H. influenzae (p<0.0001) were cultured more frequently in PCD, with P. aeruginosa (p=0.03) and Aspergillus fumigatus (p=0.001) being more frequent in CF.
Comparing TS and sputum microbiota
Sixteen patients had paired sputum and TS samples (online supplemental figures S5 and S6). There was no significant difference in alpha or beta diversity or genera. A subanalysis of longitudinal data was performed using sputum samples only. Although there was a trend towards greater relative abundance of Pseudomonas in sputum (mean 4.0% (SD 13.5%)) in sputum c.f. 0.5% (SD 0.8%) in TS, Padj>0.05), overall the trends in the most common genera, alpha and beta diversity remained unchanged (online supplemental file 1). Both TS and sputum were therefore used for longitudinal comparisons of PCD with CF. TS was analysed, where available, and sputum if no TS sample had been collected at that timepoint.
Differences in the airway microbiota between PCD and CF
Streptococcus was the most common genus in both PCD and CF (31.3% and 55.0% of total reads, respectively, p<0.0001, Padj<0.0001) (figure 1, online supplemental figure S7 and table 3). Haemophilus was notably more common in PCD (20.5%) than CF (3.2%, p<0.0001, Padj<0.0001). Pseudomonas was the second most common genus in CF (8.1%) but was less prevalent in PCD (1.2%, p=0.0001, Padj=0.01). Other common genera were similar between PCD and CF except Staphylococcus which was uncommon (<0.1%) in PCD (p=0.015, Padj>0.05).
Concordance between bacterial cultures and molecular genus identification was better with PCD than with CF (tables 2 and 3). Haemophilus was the most commonly cultured organism in PCD and showed moderate agreement with molecular identification (Cohen’s kappa 0.43). Pseudomonas was the most commonly cultured organism in CF and showed fair agreement with molecular identification (Cohen’s kappa 0.38). Alpha diversity was higher in PCD than CF (PCD, Shannon diversity median 2.12 (range 0.190–3.137) and 1.94 (CF, range 0.167–3.158), p=0.04) (figure 2). Combining all samples (all patients and timepoints), diagnosis accounted for 5.5% of variance in community structure (p=0.001, online supplemental figure S8).
Changes in the airway microbiota with age
In PCD, in the majority of patients, Streptococcus had a high relative abundance in early childhood in contrast to relative abundance of Haemophilus that showed a low relative abundance (66.7% vs 1.3% at 0.5 years, online supplemental figure S9). With age, there was an inverse relationship between the relative abundances of Streptococcus and Haemophilus: Streptococcus decreased until 5 years of age (17.2%, p=0.003) while Haemophilus increased (55.8%, p=0.005). The reverse was seen between 6 and 9 years of age. Streptococcus then decreased in relative abundance between the ages of 10 and 17 years (10.2%, p=0.03 at 17 years) while Haemophilus increased (79.3%, p=0.003 at 17 years). The relative abundance of Neisseria fluctuated peaking at 12 years of age (relative abundance 0.1% and 17% at 0.5 and 12 years, respectively, p<0.0001). No significant changes were seen in the relative abundances of Prevotella or Veillonella. There were no significant changes in the relative abundance of the five most common genera with age in CF (p>0.05) (figure 3).
In CF, at OTU level two OTUs were found to have a small (r≤0.3) but significant (Padj<0.05) change in relative abundance with age: Haemophilus (OTU ID 7005) decreased with age (r=−0.254, Padj=0.011) while Actinomyces (OTU ID 2413) increased (r=0.237, Padj=0.046). In PCD, two OTUs changed significantly with age: Streptococcus (OTU ID 10797), decreasing with age (r=−0.323, Padj=0.002) and Haemophilus (OTU ID 5932), increasing (r=0.244, Padj=0.011).
Only alpha diversity changed significantly with age (both diseases). Richness increased in early childhood, peaking at age 7 years for PCD (mean richness 33.5 at 0.5 years, 67.5 at 7 years, p=0.006) and age 4 years for CF (mean richness 29 at 0.5 years, 68 at 4 years, p=0.034, figure 4). There was no significant difference in beta diversity with age (p>0.05) for either group.
From examining individual patient barplots (online supplemental figure S9), while the airway microbiota was highly individual, Streptococcus were abundant throughout for both CF and PCD. Pseudomonas was abundant in several children with CF aged above 8 years. The changes in relative abundance of Haemophilus in PCD at an individual level were consistent with the age-related changes described overall for PCD above. There were no consistent changes in genera seen with changes in symptoms, spirometry, growth on bacterial cultures or antibiotic use. There was no apparent skewing by, for example, an individual who contributed many samples to the study.
Influence of clinical variables, pulmonary exacerbations and antibiotics
The airway microbiota was highly individual, with the patient sampled accounting for 37% (PCD) and 39% (CF) of variance in community structure (p≤0.01).
For PCD, growths of H. influenzae accounted for 5.7% of variance in community structure (p=0.001). Alpha diversity was reduced in H. influenzae culture positive samples compared with those that were culture negative (median Inverse Simpson’s diversity index 3.23 (range 1.10–15.9) and 5.16 (range 1.11–19.04), respectively, p=0.009). Gender had a weak relationship with community structure (r2=0.046, p=0.001) but none with alpha diversity (p>0.05). There was no significant relationship between alpha and beta diversity and lung function (FEV1 or forced vital capacity % predicted) for either disease.
For CF, all alpha diversity indices were higher in patients who were intermittently infected with P. aeruginosa infection compared with those who had never grown or had chronic P. aeruginosa at baseline (n=6 in each group, p=0.02, online supplemental table S3). Growth of P. aeruginosa during the study associated with reduced alpha diversity (p=0.01). Alpha diversity was higher in heterozygous p.Phe508del patients with CF than those who were homozygous or had other CFTR mutations (p=0.009). In the combined PERMANOVA, only P. aeruginosa chronic infection status had a small but significant relationship with community structure (r2=0.017, p=0.014).
Fourteen patients with PCD and 11 patients with CF experienced a pulmonary exacerbation (necessitating intravenous antibiotics) of whom 12 PCD and 7 patients with CF had sequential samples collected at baseline (B), exacerbation (E), treatment (T) and recovery (R) as previously described.16 Significant differences were seen only in PCD (see online supplemental file 1 and online supplemental figures S10 and S11).
There were no significant differences with oral or intravenous treatment antibiotic courses, prophylactic or nebulised antibiotics for either condition.
Here, we report for the first time the development of the airway microbiota in children with PCD, and compare it to that in a similar but more severe disease, CF.
Our study shows that the airway microbiota is highly individual but differs between groups of children with PCD and CF, a novel finding. Several previous studies in CF have also demonstrated high interpatient variability in the airway microbiota,6 18 strengthening arguments for personalised medicine. There was greater diversity in PCD than CF with a significant difference in both alpha and beta diversity. Since most patients with PCD studied had genotypes associated with a milder phenotype, this could suggest increased diversity of the microbiota is associated with milder disease. The significance of this is unclear, however, given that the median FEV1% and hence one marker of disease severity was similar between both groups.
Interpretation of diversity with disease severity is complex. Changes in diversity reflect changes in the relative abundance of organisms. Samples with an even spread of different organisms have greater diversity whereas those dominated by an individual organism have lower diversity. Therefore, changes in diversity may reflect presence or absence of a dominant pathogen, which drives disease severity.
There was no significant relationship between diversity (alpha or beta) and spirometry for either disease. One interpretation is that diversity of the microbiota does not influence disease severity. Disease severity in both groups was however relatively mild, reducing the ability to detect such a relationship. Furthermore, there was no significant difference in FEV1 between PCD and CF with the median FEV1 80% predicted in both groups. This is slightly lower than the median for our centre (RBH) from the UK CF Registry data for that year (FEV1 86.5% predicted in 2013) and a selection bias cannot be excluded. Thus, whether differences in the microbiota between PCD and CF relate to differences in disease prognosis could not be fully determined. FEV1, however, is not a sensitive marker for tracking particularly early lung disease in children36 and may be difficult under 5 years of age. In future studies, relating more sensitive tests of distal airway disease such as lung clearance index, forced oscillation or imaging to airway microbiota diversity could potentially be more revealing.
An inverse relationship was seen between Streptococcus and Haemophilus with age only in PCD. This trend parallels that seen in CF NBS infants in the first 2 years of life.16 Notably the microbiota in PCD and CF NBS appears to have greater similarities than with older children with CF. We speculate the microbiota in PCD is like that of the early microbiota in CF. Whether this is of direct pathological significance, or a marker of another underlying disease process is unclear.
Previous cross-sectional studies in CF identified an increase in diversity during childhood, at least until school age,4 and a decrease in adulthood.6 7 In our study, with the exception of species richness (the number of different organisms), there was no significant difference with age in either alpha or beta diversity in either disease. Many of the children in this study were on prophylactic antibiotics (81% in CF and 58% in PCD). While a treatment effect of prophylactic antibiotics was not seen in either group, given the high proportion of patients receiving prophylaxis, particularly in CF, it is possible that this suppressed changes in the microbiota over time. There were significant differences, however, at genera level: Streptococcus was the most common genus in both groups (31% PCD and 55% CF); Haemophilus was sixfold more abundant in PCD than CF (21% vs 3.2%), whereas Pseudomonas was the second most common genus (8.1%) in CF but not PCD. Several anaerobes were prevalent in PCD as also reported for CF here and elsewhere.
There was reduced diversity with growth of H. influenzae in PCD and P. aeruginosa in CF, suggesting these organisms have important influences on community structure. Previous studies in PCD have demonstrated that while H. influenzae is the most common organism cultured in children, P. aeruginosa becomes the dominant pathogen with age.37 This increase in P. aeruginosa occurs later in PCD than in CF, predominantly after 30 years of age, when transition to a mucoid phenotype also occurs.38 A cross-sectional study of non-CF bronchiectasis found a competitive effect between Haemophilus and Pseudomonas; when Haemophilus were dominant, Pseudomonas were either absent or present in very low abundance and vice versa.39 Possibly the high abundance of Haemophilus in the early CF microbiota and PCD leads to a milder phenotype, with replacement of Haemophilus by Pseudomonas in CF resulting in a more severe phenotype. Interventions aimed at preventing this switch, focussing on the mechanisms whereby Haemophilus is associated with improved prognosis in CF, are needed to further explore this potential relationship and hopefully delineate new therapeutic options.
This study has several strengths. It is the first study of the airway microbiota in children with PCD followed up for over a year. The children with PCD and CF in this study were well matched both by age and disease severity and were sampled frequently (median interval 61 and 42 days in PCD and CF, respectively). This has allowed meaningful comparisons and a detailed investigation of the microbiota to be made. The longitudinal design is important to account for the high interpatient variability in the microbiota seen both in this study and previously.
One limitation is that different sampling methods (TS and sputum) were used at different timepoints. Ideally the same sample type would have been collected at all times, but this proved unfeasible. Comparison of paired TS and sputum (n=16) did not demonstrate a difference in the microbiota and repeating the longitudinal analyses with sputum samples only did not change any conclusions. The relative abundance of Pseudomonas was lower in TS than in sputum samples as in previous studies.40 41 Pseudomonas, however, remained the second most abundant organism whether analysing sputum alone or both sputum and TS combined and the overall trends in community composition and diversity remained unchanged.
While TS are an attractive candidate as a surrogate for lower airway sampling, their accuracy in representing the lower airway microbiota remains debatable. A study of 16 children with CF demonstrated that for many participants, communities were similar between oropharyngeal samples and sputum. In those samples that diverged, there was a higher relative abundance of Pseudomonas, Staphylococcus, Enterobacteriaceae and Haemophilus in sputum samples.41 Similarly, a study of 20 children with CF found high concordance between TS and sputum samples in patients who were Pseudomonas negative.40 In the current study, 94% of patients with PCD and 61% of patients with CF were either ‘free’ or had never grown Pseudomonas at baseline. This may explain why similarity was seen when comparing paired sputum and TS from the children in this study. Previously we have shown that TS correlate with the lower airway microbiota. Although differences exist precluding their use for individual decision making, TS can distinguish between disease groups and are useful for longitudinal study of the microbiota between groups of patients with CSLD.23
More children with CF had gastro-oesophageal reflux disease (GORD) requiring treatment with proton pump inhibitors (PPIs) than children with PCD. GORD and specifically treatment with PPIs may affect the lung microbiota.42 Thus, it is possible that the differences between CF and PCD in this study could relate to GORD. That said we detected no effect of treatment with PPIs.
One limitation of 16S rRNA gene sequencing is that it is often unable to discern species level changes and cannot assess functional changes. It is plausible that while overall community composition may not have changed, functional changes occurred in gene expression, metabolite production or interactions between different species with disease status.43 To this end, analysing longitudinal differences in CSLD with disease status using metagenomics or other functional approaches, as well as including digital droplet PCR to distinguish between absolute and relative abundance changes, would be an important area for future work.
In conclusion, we have demonstrated that the PCD microbiota differs from that of CF. The airway microbiota is highly individual in these diseases, further strengthening arguments for personalised approaches to patient management. Diversity was higher in PCD and community composition differed. There is a greater similarity between PCD and early CF diagnosed on NBS than with later established CF. Pseudomonas is more prevalent in CF contrasting with Haemophilus in PCD. An inverse relationship was seen between Streptococcus and Haemophilus with age in PCD but not CF. A similar relationship has been seen previously in NBS infants with CF.16 This could suggest a switch from a less pathogenic to a more pathogenic community composition later in childhood in CF, potentially influenced by a competitive relationship between Haemophilus and Pseudomonas. Further study to better understand this relationship may lead to new therapeutic approaches.
Data availability statement
Data are available in a public, open access repository. Sequence data is available at the ENA Accession number: PRJEB26618.
Patient consent for publication
This study involves human participants and was approved by the RBH Biomedical Research Unit Advanced Lung Disease Biobank (NRES reference 10/H0504/9). Participants gave informed consent to participate in the study before taking part.
Contributors BA planned the project, recruited participants, designed and performed experiments, analysed the data, wrote the manuscript and is the guarantor for the overall content; MJC planned the project, designed experiments, analysed the data and wrote the manuscript; LC and PJ designed experiments, analysed the data and wrote the manuscript; LG collected data and wrote the manuscript; WC, JD, MM and AB planned the project, designed experiments, analysed the data and wrote the manuscript. All authors reviewed, revised and approved the manuscript for submission.
Funding The authors thank Professor Claire Hogg for helpful discussions and her assistance in patient recruitment and the NIHR Biomedical Research Unit at Royal Brompton and Harefield NHS Foundation Trust. AB was supported by the NIHR Respiratory Disease Biomedical Research Unit at the Royal Brompton and Harefield NHS Foundation Trust and Imperial College London. AB is an emeritus NIHR Senior Investigator. BA was supported by NIHR. MM and WC were supported by the Asmarley Trust and Wellcome Trust. JD is an NIHR Senior Investigator.
Competing interests None declared.
Provenance and peer review Not commissioned; externally peer reviewed.
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