TY - JOUR T1 - Using a non-invasive assessment of lung injury in a murine model of acute lung injury JF - BMJ Open Respiratory Research JO - BMJ Open Resp Res DO - 10.1136/bmjresp-2013-000014 VL - 1 IS - 1 SP - e000014 AU - Siân Lax AU - Michael R Wilson AU - Masao Takata AU - David R Thickett Y1 - 2014/01/01 UR - http://bmjopenrespres.bmj.com/content/1/1/e000014.abstract N2 - Arterial oxygen saturation has not been assessed sequentially in conscious mice as a direct consequence of an in vivo murine model of acute lung injury. Here, we report daily changes in arterial oxygen saturation and other cardiopulmonary parameters by using infrared pulse oximetry following intratracheal lipopolysaccharide (IT-LPS) for up to 9 days, and following IT-phosphate buffered saline up to 72 h as a control. We show that arterial oxygen saturation decreases, with maximal decline at 96 h post IT-LPS. Blood oxygen levels negatively correlate with 7 of 10 quantitative markers of murine lung injury, including neutrophilia and interleukin-6 expression. This identifies infrared pulse oximetry as a method to non-invasively monitor arterial oxygen saturation following direct LPS instillations. ER -