RT Journal Article
SR Electronic
T1 Using a non-invasive assessment of lung injury in a murine model of acute lung injury
JF BMJ Open Respiratory Research
JO BMJ Open Resp Res
FD British Thoracic Society
SP e000014
DO 10.1136/bmjresp-2013-000014
VO 1
IS 1
A1 Siân Lax
A1 Michael R Wilson
A1 Masao Takata
A1 David R Thickett
YR 2014
UL http://bmjopenrespres.bmj.com/content/1/1/e000014.abstract
AB Arterial oxygen saturation has not been assessed sequentially in conscious mice as a direct consequence of an in vivo murine model of acute lung injury. Here, we report daily changes in arterial oxygen saturation and other cardiopulmonary parameters by using infrared pulse oximetry following intratracheal lipopolysaccharide (IT-LPS) for up to 9 days, and following IT-phosphate buffered saline up to 72 h as a control. We show that arterial oxygen saturation decreases, with maximal decline at 96 h post IT-LPS. Blood oxygen levels negatively correlate with 7 of 10 quantitative markers of murine lung injury, including neutrophilia and interleukin-6 expression. This identifies infrared pulse oximetry as a method to non-invasively monitor arterial oxygen saturation following direct LPS instillations.