RT Journal Article
SR Electronic
T1 Feasibility study of in vitro drug sensitivity assay of advanced non-small cell lung adenocarcinomas
JF BMJ Open Respiratory Research
JO BMJ Open Resp Res
FD British Thoracic Society
SP e000505
DO 10.1136/bmjresp-2019-000505
VO 7
IS 1
A1 Emoke Papp
A1 Anita Steib
A1 Elhusseiny MM Abdelwahab
A1 Judit Meggyes-Rapp
A1 Laszlo Jakab
A1 Gabor Smuk
A1 Erzsebet Schlegl
A1 Judit Moldvay
A1 Veronika Sárosi
A1 Judit E Pongracz
YR 2020
UL http://bmjopenrespres.bmj.com/content/7/1/e000505.abstract
AB Background Despite improved screening techniques, diagnosis of lung cancer is often late and its prognosis is poor. In the present study, in vitro chemosensitivity of solid tumours and pleural effusions of lung adenocarcinomas were analysed and compared with clinical drug response.Methods Tumour cells were isolated from resected solid tumours or pleural effusions, and cryopreserved. Three-dimensional (3D) tissue aggregate cultures were set up when the oncoteam reached therapy decision for individual patients. The aggregates were then treated with the selected drug or drug combination and in vitro chemosensitivity was tested individually measuring ATP levels. The clinical response to therapy was assessed by standard clinical evaluation over an 18 months period.Results Based on the data, the in vitro chemosensitivity test results correlate well with clinical treatment response.Conclusions Such tests if implemented into the clinical decision making process might allow the selection of an even more individualised chemotherapy protocol which could lead to better therapy response.