RT Journal Article
SR Electronic
T1 Novel non-invasive particles in exhaled air method to explore the lining fluid of small airways—a European population-based cohort study
JF BMJ Open Respiratory Research
JO BMJ Open Resp Res
FD British Thoracic Society
SP e000804
DO 10.1136/bmjresp-2020-000804
VO 8
IS 1
A1 Laith Hussain-Alkhateeb
A1 Björn Bake
A1 Mathias Holm
A1 Össur Emilsson
A1 Ekaterina Mirgorodskaya
A1 Anna-Carin Olin
YR 2021
UL http://bmjopenrespres.bmj.com/content/8/1/e000804.abstract
AB Introduction Respiratory tract lining fluid of small airways mainly consists of surfactant that can be investigated by collection of the particles of exhaled aerosol (PExA) method. This offers an exciting prospect to monitor small airway pathology, including subjects with asthma and smokers.Aim To explore the influence of anthropometric factors and gender on phospholipids, surfactant protein A (SP-A) and albumin of the lining fluid of small airwaysand to examine the association with asthma and smoking. Furthermore, to examine if the surfactant components can predict lung function in terms of spirometry variables.Method This study employs the population-based cohort of the European Community Respiratory Health Survey III, including participants from Gothenburg city, Sweden (n=200). The PExA method enabled quantitative description and analytical analysis of phospholipids, SP-A and albumin of the lining fluid of small airways.Results Age was a significant predictor of the phospholipids. The components PC14:0/16:0, PC16:0/18:2 (PC, phosphatidylcholine) and SP-A were higher among subjects with asthma, whereas albumin was lower. Among smokers, there were higher levels particularly of di-palmitoyl-di-phosphatidyl-choline compared with non-smokers. Most phospholipids significantly predicted the spirometry variables.Conclusion This non-invasive PExA method appears to have great potential to explore the role of lipids and proteins of surfactant in respiratory disease.