Constitutive and stimulated cytokine production by primary nasal epithelial cells
| Stimulant | ||||||
|---|---|---|---|---|---|---|
| Basal | Staphylococcus aureus PGN | S. aureus LTA | Pseudomonas aeruginosa LPS | CpG | TNF | |
| IL-1β (pg/mL) | 7.1 0–18.7 | 7.7 0–23.8 | 4.2 0–21.9 | 3.6 0–16.4 | 6 0–17.3 | 8.1 0–165 |
| IL-6 (pg/mL) | 29.7 13.7–313 | 140 21.6–695 | 52.1 6.3–459 | 139 7.9–279 | 45 4.7–535 | 956 ** 67.5–3173 |
| IL-8 (pg/mL) | 504 192–1557 | 1363 378–3821 | 663 297–2309 | 740 131–4295 | 520 11.8–2531 | 7817 *** 2033–48 688 |
| IL-10 (pg/mL) | 9.2 4–18.7 | 12.5 4–21.6 | 7.1 0–14.5 | 6.4 0–18.6 | 6.5 0–21.1 | 13 0–67 |
| IL-12 (pg/mL) | 13.2 3.6–19.8 | 12.1 0–21 | 8.8 0–16.1 | 10.3 0–21.4 | 10.4 0–26.7 | 10.4 0–23.3 |
| TNF (pg/mL) | 10 1.7–15 | 6.2 2–24.3 | 7.2 0–11.8 | 6.5 3–16.1 | 6.3 0–17.5 | † |
Data are expressed as median (upper line, italic) and range (lower line, normal text). n=6 for all conditions. PGN and LTA were applied at 10 μg/mL, LPS at 100 ng/mL, CpG at 1 μM and TNF at 10 ng/mL. Statistical analysis was by Friedman's test and Dunn's post hoc test. *p<0.05, **p<0.01, ***p<0.001 relative to basal levels, by Dunn's post hoc test.
†TNF was used as a positive control; TNF was not measured in TNF-stimulated cells.
IL, interleukin; LPS, lipopolysaccharide; LTA, lipoteichoic acid; TNF, tumour necrosis factor; PGN, peptidoglycan.