Ex vivo monocyte responsiveness to stimulation with LPS
| Cytokine | 0 h | 6 h | 24 h | 48 h | ANOVA p Value |
|---|---|---|---|---|---|
| pg/mL/μg | pg/mL/μg | pg/mL/μg | pg/mL/μg | ||
| Median (IQR) | Median (IQR) | Median (IQR) | Median (IQR) | ||
| IL-8 | 1334 (915–1923) | 2325 (1194–3059)** | 2177 (1072–2734) | 2409 (1420–2787)** | 0.034 |
| IL-6 | 282 (130–362) | 391 (170–580) | 395 (146–601) | 454 (192–703)* | 0.358 |
| TNF | 39.2 (25.8–49.6) | 40.7 (25.4–69.5) | 43.6 (23.8–67.8) | 48.1 (34.8–82.4)* | 0.028 |
| IL-10 | 2.14 (1.18–3.52) | 2.61 (0.88–5.07) | 1.96 (1.02–3.36) | 1.63 (0.78–2.77)* | 0.122 |
| IL-1β | 28.1 (15.5–52.6) | 20.1 (11.6–35.1) | 23.4 (11.1–73.5) | 18.7 (10.8–32.7) | 0.291 |
| IL-12 | 0.17 (0.14–0.26) | 0.18 (0.12–0.24) | 0.19 (0.13–0.26) | 0.16 (0.13–0.26) | 0.451 |
Blood monocytes were isolated at each of the four perioperative time points and stimulated with LPS for 18 h, ie, isolated monocytes were stimulated once with LPS—no cells received repeated application of LPS. Cytokine concentrations in supernatants were quantified. Data were adjusted for total protein content. Data are presented as medians and IQR. Data were analysed for trend by ANOVA; individual time points were assessed relative to the preoperative value by paired Student t test, *p<0.05, **p<0.005. n=40 for all time points except 48 h, where n=38.
ANOVA, analysis of variance; LPS, lipopolysaccharide; TNF, tumour necrosis factor.