Biochemical and Biophysical Research Communications
Adiponectin induces the anti-inflammatory cytokines IL-10 and IL-1RA in human leukocytes☆
Section snippets
Materials and methods
Cell lines. Human THP1 cells were routinely maintained in RPMI-1640 supplemented with 10% FCS.
Preparation of monocytes, macrophages, and dendritic cells. Human monocytes were isolated from peripheral blood. Briefly, mononuclear cells (PBMC) were isolated from freshly collected buffy-coat preparations of whole human blood by Ficoll–Hypaque gradient centrifugation. CD14+ cells were selected by magnetic bead separation (purity >95%).
For the preparation of macrophages CD14+ cells were seeded grown
IL-10 and IL-1RA are induced in adiponectin-treated monocytes
Incubation of primary human monocytes with adiponectin significantly induced the production of the anti-inflammatory cytokines IL-10 and IL-1RA (Figs. 1A and B). In LPS-stimulated cells, the production of IL-10 was further enhanced by adiponectin, whereas the production of IL-1RA decreased in this setting. Adiponectin treatment of the myeloid cell line THP1 resulted in increased IL-1RA levels in both, unstimulated as well as LPS-activated, cells whereas IL-10 was not detectable (data not shown).
Discussion
Our current study demonstrates that the adipokine adiponectin is sufficient to induce the expression of anti-inflammatory cytokines such as IL-10 and IL-1RA in a variety of myeloid cell types, such as monocytes, a myeloid cell line (THP-1), primary human dendritic cells as well as monocyte-derived macrophages. In line with this anti-inflammatory phenotype, adiponectin-treated macrophages are characterized by a reduced production of IFN-γ as well as a limited ability to evoke allogeneic T-cell
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This work was supported by the FWF Grant No. P15783 (to H.T.).