Original articleDetection of Mycobacterium tuberculosis Genome in Vitreous Fluid of Eyes with Multifocal Serpiginoid Choroiditis
Section snippets
Methods
We prospectively enrolled 11 patients (11 eyes) with active MSC in 1 or both eyes between October 2012 and December 2013 using following inclusion criteria: (1) evidence of active MSC lesions with central healing and active edges that showed early hypofluorescence and late hyperfluorescence on fluorescein angiography in at least 1 eye; (2) presence of significant vitreous cells (2+ or more) in the affected eye with or without anterior segment inflammation; (3) documented positive (10 mm of
Results
During the study period, 13 patients who met the inclusion criteria were offered the option of PPV. Of these, 2 patients declined. There were 8 males and 3 females. The mean age was 28.1±10.18 years (range, 15–50 years). The disease was bilateral in 5 patients. Nine patients had positive TST or QuantiFERON-TB Gold In-Tube test results. The multitargeted PCR results were positive in 10 eyes (Fig 2). Of the 10 eyes with positive PCR results, rpoB gene sequencing for rifampicin resistance detected
Discussion
This is the first study of diagnostic PPV demonstrating the presence of M. tuberculosis DNA in the vitreous fluid of eyes with MSC. In this series, different molecular techniques established a definitive diagnosis of tuberculosis, such as multitargeted PCR analysis in 10 of 11 patients (previously reported sensitivity of 77.8%),10 the MTBDRplus assay in 6 cases, and the Gene Xpert MTB/RIF assay in 4 cases.
Multifocal serpiginous choroiditis presents as an isolated ocular disease, and definitive
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Financial Disclosure(s): The author(s) have no proprietary or commercial interest in any materials discussed in this article.
Supported by the Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi, India.
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Both Dr. Bansal and Dr. Sharma contributed equally as first authors.