Short communicationCharacterization of mesenchymal stem cells (MSCs) from human fetal lung: Potential differentiation of germ cells
Introduction
Mesenchymal stem cells (MSCs) are multipotent progenitor cells with the capacity to differentiate into many types of tissue cells (Pittenger et al., 1999, Hennrick et al., 2007, Young et al., 2001). Bone marrow is believed to be the enriched reservoir of MSCs and major source for these precursor cells that populate other adult tissues and organs (Prockop, 1997, Majumdar et al., 1998). MSCs can be isolated from circulating blood (Zvaifler et al., 2000), as well as from diverse nonhematopoietic tissues and connective tissues such as synovium (DeBari et al., 2001), adipose tissue (Zuk et al., 2001), trabecular bone (Noth et al., 2002), dermis (Young et al., 2001, Hoogduijn et al., 2007), dental pulp (Pierdomenico et al., 2005), and the lung (Sabatini et al., 2005). There have been no reports of MSCs that have been conclusively demonstrated to be of tissue-specific origin in any adult organ system, including the lung (Lama et al., 2007). The origins of mesenchymal cells that participate in normal and abnormal repair responses in the human adult lung remain unclear. In this study, we isolate the multipotent MSCs from the human fetal lung and investigate the ability to differentiate into dipocytes and osteoblasts, even cardiac cells, neural cells and sperm like cells.
Section snippets
Samples
The study was approved by the patient and the biological ethical committee of Shaanxi Centre of Stem Cells Engineering & Technology. The lung were mechanically collected, washed with PBS again, dissected and incubated with 0.125% trypsin and 0.02% EDTA for 10–20 min or with 0.125% collagenase IV for 20–40 min at 37 °C, then gently pipetted, centrifuged at 1000 rpm for 5–10 min. Cells were seeded at a density of 1 × 105 mononuclear cells per microliter and transferred to medium containing 10% FCS
Characterization of hMSCs derived human fetal lung
Isolation of plastic-adherent fibroblastoid cells from human fetal lung. MSCs have been well recognized to derive from single-cell suspensions of bone marrow by the selective growth of plastic-adherent fibroblast-like cell colonies in liquid culture medium. Within 2–5 passages after the initial plating of the primary culture, hMSCs derived human fetal lung appeared as a monolayer of large, flat, and spindle-shaped cells (Fig. 1A). As the cells approached confluence, they assumed a more
Discussion
In this study, we investigated the possibility to isolate fibroblast-like cells from human fetal lung and these cells exhibited several characteristics typical of classical bone marrow derived MSCs (Pittenger et al., 1999, Short et al., 2003, Majumdar et al., 1998). Firstly, they can be isolated by adherence purification on tissue culture plastic and form distinct CFU-Fs in vitro culture; secondly, these cells express surface proteins typically associated with MSCs but do not express
Acknowledgments
We are grateful to Professor Huayan Wang for discussion on the manuscript. This work was supported by grants from the Program (30200137) from National Natural Science Foundation of China, Key Program of State Education Ministry (109148), The Scientific Research Program of Shaanxi Province (2008K02-05), China Postdoctoral Science Foundation funded project (20080431253, 200801438), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (14110101)
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